In the present study, copper oxide (CuO) colloidal nanoparticles (NPs) were synthesized using laser
ablation of copper pellet immersed in deionized water. Pulsed
Nd: YAG laser was used to irradiate the targets at different
laser energies and various ablation times. NP suspensions
were characterized by UV–Vis spectroscopy, XRD, and
TEM. The absorption spectrum exhibited a peak at ∼275nm
and another peak with low intensity at ∼645nm. The XRD
pattern of the NPs proved the presence of (–111) and (112)
planes assigned to the CuO phase. The TEM images showed
nearly spherical shape nanoparticles CuO NPs with size of
3–40nm. The antibacterial activity of CuO NPs was first
carried out against four types of bacteria: Escherichia coli,
Pseudomonas aeruginosa, Proteus vulgaris and Staphylococcus aureus, by liquid medium method. CuO NPs showed
the highest antibacterial activity against E. coli at the highest
concentration (1000 µg mL−1). CuO NPs and amoxicillin
had a synergistic effect on inhibiting E. coli and S. aureus
growth; this effect was also tested using the well diffusion
method. In this method, CuO NPs at a concentration of
1000 µg mL−1 along with amoxicillin showed the inhibition
zone against E. coli (26.0 ± 1.00 mm), as well as complete
inhibition of bacteria against S. aureus.
Keywords CuO · Nanoparticles · PLAL · X-ray
diffraction · TEM · Antibacterial activity
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